Carnosine-Modified Fullerene as a Highly Enhanced ROS Scavenger for Mitigating Acute Oxidative Stress

Fullerenes are known as highly efficient scavengers for reactive oxygen species (ROSs). In this study, a carnosine-modified fullerene derivative (C₆₀-Car) was synthesized via a one-step nucleophilic addition reaction. C₆₀-Car forms nanoparticles (NPs) readily in water at neutral pH and room temperature through self-assembly. The C₆₀-Car NPs were found to possess good water solubility, biocompatibility, and excellent ROSs scavenging capability. The scavenging efficiency of ROSs is as high as 92.49% and significantly better than that of hydroxyfullerene (C₆₀-OH NPs, 70.92%) and l-carnosine. Furthermore, C₆₀-Car NPs showed strong cytoprotective ability against H₂O₂-induced damage to the normal human fetal hepatocyte cells (L-02) and human epidermal keratinocytes-adult (HEK-a) cells at a lower concentration of 2.5 μM. In contrast, C₆₀-OH NPs showed a minor cytoprotective effect on cells at a high concentration of 10 μM. The excellent properties of such a fullerene derivative, C₆₀-Car, can be attributed largely to the involvement of l-carnosine with biological activity and antioxidant property, which make it better for biomedicine, and it may provide a new strategy for mitigating acute oxidative stress based on fullerene materials.