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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
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Regulating Divergent Transcriptomes through mRNA Splicing and Its Modulation Using Various Small Compounds.
Ken-Ichi Fujita1, Takaki Ishizuka1, Mizuki Mitsukawa1
1Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
International Journal of Molecular Sciences
|March 20, 2020
Summary
Alternative splicing creates diverse human transcriptomes, regulating gene expression. Disruptions cause diseases like cancer, but splicing inhibitors offer therapeutic potential.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Human transcriptomes exhibit significant divergence, primarily due to alternative splicing of messenger RNA (mRNA).
- Alternative splicing, influenced by spliceosomal factors and RNA structures like G-quadruplexes, generates transcript diversity and regulates gene expression.
- Aberrant splicing is implicated in severe conditions, including cancer and neurodegenerative diseases.
Purpose of the Study:
- To review the regulation of divergent transcriptomes via alternative mRNA splicing.
- To elucidate the link between splicing dysregulation and disease pathogenesis.
- To explore compounds modulating mRNA splicing for research and therapeutic applications.
Main Methods:
- Review of existing literature on alternative splicing mechanisms.
- Analysis of the relationship between splicing defects and disease.
- Examination of splicing inhibitors and their therapeutic potential.
Main Results:
- Alternative splicing is a key driver of transcriptome complexity and gene regulation.
- Splicing dysregulation is a significant factor in various human diseases.
- Splicing inhibitors, including food-derived compounds, show promise as research tools and anticancer agents.
Conclusions:
- Understanding alternative splicing is crucial for comprehending gene expression and disease.
- Modulators of mRNA splicing represent a promising avenue for therapeutic development.
- Further research into splicing-modulating compounds could advance cancer treatment and basic science.
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