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[Effect of adenosine on P50 and its relation to 2,3-diphosphoglycerate]
P Silva Orrego1, T Vicente Vera, F Amador
1Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Insights
Adenosine administration significantly alters the haemoglobin dissociation curve (DHC) in dogs, generally shifting it leftward. This effect correlates with changes in intraerythrocytic 2,3-diphosphoglyceric acid (2,3-DPG) levels.
Area of Science:
- Physiology
- Biochemistry
Context:
- Investigates the physiological impact of adenosine on oxygen transport.
- Examines the relationship between adenosine, haemoglobin function, and red blood cell metabolism.
Purpose:
- To determine how intravenous adenosine doses affect the haemoglobin dissociation curve (DHC).
- To explore the correlation between DHC shifts and intraerythrocytic 2,3-diphosphoglyceric acid (2,3-DPG) levels.
Summary:
- Adenosine administration in anesthetized dogs caused significant leftward shifts in the DHC, except at a high dose (120 µg/kg/min) which induced a rightward shift.
- These DHC alterations were observed to be parallel to changes in intraerythrocytic 2,3-DPG concentrations.
- The study concludes that adenosine modulates the DHC, primarily via alterations in 2,3-DPG levels.
Impact:
- Provides insights into the mechanism of adenosine's effect on oxygen delivery.
- Highlights the role of 2,3-DPG in mediating adenosine-induced haemoglobin changes.
- Contributes to understanding haemoglobin-oxygen binding dynamics in response to pharmacological agents.
Abstract:
The effects of increasing doses of intravenous adenosine upon the dissociation haemoglobin curve (DHC) and its relation to the intraerythrocytic level of 2,3-diphosphoglyceric acid (2,3-DPG), were studied in 17 anesthetized dogs. The DHC moved significantly to the left in all dogs except at the dose of 120 micrograms/kg/min which induces a displacement to the right. These changes in the DHC were parallel to the intraerythrocytic levels of 2,3-DPG. We conclude that adenosine modifies the DHC, shifting it generally to the left, and that this effect seems to be related to a change in the intraerythrocytic level of 2,3-DPG.