Runners' metabolomic changes following marathon

Area of Science:

• Human metabolomics
• Exercise physiology
• Biochemistry

Background:

• Marathon running is a popular aerobic exercise with limited understanding of its holistic impact on the serum metabolome.
• Investigating metabolic changes post-marathon is crucial for athlete performance and health.

Purpose of the Study:

• To comprehensively analyze the effects of marathon running on human serum metabolomics.
• To explore the interrelationships among various metabolites before and after a marathon.

Main Methods:

• Serum samples from 20 male marathon runners were collected pre- and post-race.
• Untargeted two-dimensional gas chromatography time-of-flight mass spectrometry was used for metabolite identification.

Main Results:

• Significant increases in serum urea, creatine kinase, cortisol, and various metabolites including pyruvic acid, glyceric acid, and lipid indicators were observed.
• Significant decreases in serum testosterone, glucosamine, and certain amino acids like serine and valine were noted.
• Correlations were found between marathon performance, body composition, VO2max, hemoglobin, and serum testosterone/cortisol levels.

Conclusions:

• Marathon completion enhances glucose and lipid metabolism in athletes.
• Glucosamine metabolism decreases, while caffeine metabolism increases post-marathon.
• The study provides novel insights into marathon performance and the nutritional status of athletes.