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Updated: Dec 26, 2025

Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements
Celso-Afonso Klein-Junior1, Roberto Zimmer2, Diana-Lina-Bronca Borghetti2
1Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Cachoeira do Sul, Rio Grande do Sul, Brazil.
Background:
The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements.
Material And Methods:
The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to the MTT test. Data were analyzed using analysis of variance (ANOVA) followed by post-hoc Student-Newman-Keuls (<0.05).
Results:
The results indicated significant differences between the materials according to their composition, i.e., light-cured cements treated with a jet of warm air showed similar cytotoxicity levels to those observed for conventional cement, suggesting that they may be considered alternatives in cases requiring pulp-capping treatment.
Conclusions:
Application of a hot air stream reduced cytotoxicity of materials tested. Key words:Dental pulp capping, dental cements, calcium hydroxide, cell survival.
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