Related Experiment Video
Updated: Dec 26, 2025
Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle
Published on: August 30, 2021
Staging of the estrous cycle and induction of estrus in experimental rodents: an update
Ayodeji Folorunsho Ajayi1, Roland Eghoghosoa Akhigbe1
1Department of Physiology, College of Medicinek, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.
Background:
Determination of the phases of the estrous cycle and induction of estrus (heat) in experimental animals remains useful, especially in reproductive function research.
Main Body Of The Abstract:
This review provides a detailed description and discusses extensively the variations observed in different phases of the estrous cycle in laboratory animals using rats and mice as examples. It also illustrates how these phases can be determined and how to induce estrus 'heat' when required. The phases of the estrous cycle can be determined using various methods such as visual assessment, vaginal smear/cytology, histology of female reproductive organs (vagina, uterus and ovaries), vaginal wall impedance assessment and determination of urine biochemical parameters. Female animals can be artificially brought to estrus phase 'heat' to make them receptive to male counterparts.
Conclusion:
Determination of the length and phases of the estrous cycle and induction of estrus are useful in teaching and research and evaluating the effects of drugs/chemicals on the reproductive functions.
More Related Videos
09:01Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
Published on: September 15, 2012
11:04Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
Published on: September 3, 2020