Toxicity of environmental ozone exposure on mice olfactory bulbs, using Western blot technique

Environmental ozone (O₃) exposure has adverse effects on different body systems. This experimental work aimed to study the effect(s) of O₃ exposure on the olfactory bulbs (OB) of Swiss Webster and C57BL/6J mouse strains, using Western blot technique. Both mice strains were exposed to different O₃ doses for different number of exposures and durations. The results indicated that O₃ exposure caused a significant increase in the level of the proteins involved in the oxidative stress state such as 4-hydroxynonenal (4HNE) and Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), in addition to the total OB proteins in Swiss Webster mouse strain. However, this effect was not observed in C57BL/6J mouse strain. Furthermore, CYP1A1 was completely absent in the Green fluorescent protein (GFP) C57BL/6J O₃ exposed mice. Moreover, O₃ exposure caused a significant decrease in the body weight of the tested mice from the two strains.