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Circ0004390 promotes cell proliferation through sponging miR-198 in ovarian cancer

Fei Xu1, Mengdong Ni1, Jiajia Li1

  • 1Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Biochemical and Biophysical Research Communications
|March 21, 2020
PubMed

Insights

Circular RNAs (circRNAs) are implicated in ovarian cancer. Researchers identified Circ0004390, upregulated in tumors, which promotes proliferation by sponging miR-198 to regulate MET, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging as critical regulators in human diseases, including cancer.
  • The specific roles of circRNAs in ovarian cancer pathogenesis remain largely unexplored.
  • This study investigates the function of circRNAs in ovarian cancer progression.

Purpose of the Study:

  • To identify differentially expressed circRNAs in ovarian cancer tissues.
  • To characterize the function and mechanism of a novel circRNA, Circ0004390, in ovarian cancer.
  • To evaluate Circ0004390 as a potential therapeutic target for ovarian cancer.

Main Methods:

  • Differential expression analysis of circRNAs in ovarian cancer and normal tissues.
  • Functional assays including knockdown of Circ0004390 to assess its impact on cell proliferation.
  • Mechanism investigation involving RNA immunoprecipitation and luciferase reporter assays to elucidate the interaction between Circ0004390, miR-198, and MET.
  • Correlation analysis between Circ0004390 levels and patient survival data.

Main Results:

  • A significant number of circRNAs were found to be differentially expressed in ovarian cancer tissues.
  • Circ0004390, derived from the LPAR3 gene, was frequently upregulated in ovarian cancer tissues.
  • Knockdown of Circ0004390 significantly inhibited ovarian cancer cell proliferation.
  • Circ0004390 acts as a molecular sponge for the miR-198 family, thereby regulating MET expression.
  • Higher levels of Circ0004390 correlated with poorer overall survival in ovarian cancer patients.

Conclusions:

  • Circ0004390 plays a crucial role in promoting ovarian cancer cell proliferation.
  • The Circ0004390/miR-198/MET axis is a key regulatory pathway in ovarian cancer.
  • Circ0004390 represents a promising diagnostic biomarker and therapeutic target for ovarian cancer treatment.
Keywords:
METOvarian cancercirc_0004390miR-198

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