Related Experiment Video

Updated: Dec 25, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K

Long non-coding (lnc)RNA PAPAS overexpression inhibits tumor growth in papillary thyroid carcinoma by downregulating

Juan Xiao1, Zhaowei Bing1, Ge Xiao2

  • 1Department of Endocrinology, Qingdao Municipal Hospital, Qingdao, Shandong 266000, P.R. China.

Oncology Letters
|March 21, 2020
PubMed

Insights

Long non-coding RNA PAPAS inhibits papillary thyroid carcinoma (PTC) growth by downregulating HOTTIP. PAPAS is downregulated, while HOTTIP is upregulated in PTC, suggesting PAPAS

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play crucial roles in cellular processes.
  • The lncRNA pre-ribosomal RNA antisense transcripts (PAPAS) is known to repress ribosomal RNA synthesis.
  • The specific role of PAPAS in human diseases, particularly cancer, remains largely unexplored.

Purpose of the Study:

  • To investigate the functional role of PAPAS in the development and progression of papillary thyroid carcinoma (PTC).
  • To elucidate the relationship between PAPAS and another lncRNA, HOTTIP, in the context of PTC.

Main Methods:

  • Quantitative real-time PCR was used to assess PAPAS and HOTTIP expression levels in PTC tissues and cell lines.
  • Functional assays, including cell proliferation and cell cycle analysis, were performed following PAPAS and HOTTIP overexpression or knockdown.
  • Western blotting was employed to examine protein expression changes.

Main Results:

  • PAPAS expression was significantly downregulated in PTC tissues compared to normal thyroid tissues.
  • HOTTIP expression was inversely correlated with PAPAS expression in PTC patients.
  • Overexpression of PAPAS inhibited PTC cell proliferation and downregulated HOTTIP expression, suggesting PAPAS acts upstream of HOTTIP.
  • Conversely, HOTTIP overexpression promoted PTC cell proliferation and partially reversed the inhibitory effects of PAPAS overexpression.

Conclusions:

  • lncRNA PAPAS functions as a tumor suppressor in papillary thyroid carcinoma.
  • The tumor-suppressive role of PAPAS in PTC is mediated, at least in part, by the downregulation of lncRNA HOTTIP.
  • PAPAS may serve as a potential therapeutic target for papillary thyroid carcinoma.
Keywords:
long non-coding RNA HOXA transcript at the distal tiplong non-coding RNA pre-ribosomal RNA antisense transcriptspapillary thyroid carcinomaproliferation

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.6K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.0K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
10.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K