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Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Circular RNA CircHIPK3 Promotes Gemcitabine Sensitivity in Bladder Cancer.

Fang Xie1, Ning Zhao2, Hui Zhang3

  • 1Medical Basic Experimental Teaching Center, China Medical University, Shenyang, 110122, China.

Journal of Cancer
|March 21, 2020
PubMed
Summary
This summary is machine-generated.

Circular RNA circHIPK3 is downregulated in bladder cancer (BC), correlating with poor prognosis and gemcitabine resistance. Restoring circHIPK3 enhances chemotherapy sensitivity in BC cells.

Keywords:
bladder cancerchemotherapycircHIPK3circular RNAgemcitabine

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their regulatory roles in cancer development.
  • Chemotherapy resistance is a significant challenge in treating bladder cancer (BC).
  • The specific role of circHIPK3 in BC chemoresistance remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of circHIPK3 in the context of chemotherapy resistance in bladder cancer.
  • To determine the prognostic value of circHIPK3 in BC patients.
  • To explore the effect of circHIPK3 expression on gemcitabine sensitivity in BC.

Main Methods:

  • Quantitative real-time PCR (qPCR) to measure circHIPK3 expression levels in BC tissues and cell lines.
  • Cell Counting Kit-8 (CCK-8) assay to assess cell proliferation and gemcitabine sensitivity.
  • Flow cytometry to analyze apoptosis rates in response to gemcitabine treatment.

Main Results:

  • circHIPK3 expression was significantly downregulated in BC tissues and cell lines, particularly in gemcitabine-resistant cells.
  • Lower circHIPK3 levels correlated negatively with advanced pathological grade, lymph node metastasis, and gemcitabine insensitivity.
  • Overexpression of circHIPK3 reduced the IC50 of gemcitabine and enhanced its cytotoxic effects in resistant BC cells.

Conclusions:

  • circHIPK3 is a potential independent prognostic biomarker for bladder cancer patients.
  • Downregulated circHIPK3 is associated with gemcitabine insensitivity in BC.
  • Restoring circHIPK3 expression can promote gemcitabine sensitivity in bladder cancer.