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SET7 interacts with HDAC6 and suppresses the development of colon cancer through inactivation of HDAC6

Shi-Lan Zhang1, Xiao Du1, Lin-Na Tan1

  • 1Department of Gastroenterology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, P.R. China.

American Journal of Translational Research
|March 21, 2020
PubMed

Insights

SET7, a tumor suppressor, is downregulated in colorectal cancer (CRC) and inhibits cell proliferation and migration. SET7 interacts with HDAC6, increasing acetylated α-tubulin and suppressing tumor growth, offering a potential therapeutic target for colon cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • SET7, the first identified lysine methyltransferase, is implicated in tumorigenesis.
  • SET7 expression is often altered in various cancers, suggesting a role in tumor development.

Purpose of the Study:

  • To investigate the clinical significance of SET7 in colorectal cancer (CRC).
  • To elucidate the biological mechanisms by which SET7 influences CRC cell proliferation and migration.

Main Methods:

  • Analysis of SET7 expression in CRC tissues and correlation with patient prognosis.
  • In vitro loss-of-function and gain-of-function studies in colon cancer cells.
  • Co-immunoprecipitation assays to assess SET7-HDAC6 interaction.
  • Western blotting to evaluate levels of acetylated α-tubulin and p-ERK/ERK.

Main Results:

  • SET7 expression was significantly lower in CRC tissues compared to adjacent tissues, correlating with poor prognosis.
  • SET7 suppressed colon cancer cell proliferation and migration, partly through interaction with HDAC6.
  • SET7-HDAC6 interaction increased acetylated α-tubulin levels and decreased p-ERK/ERK ratios, suggesting tumor-suppressive activity.
  • SET7's tumor-suppressive role is mediated by increasing acetylated α-tubulin via HDAC6 and potentially inhibiting the ERK signaling pathway.

Conclusions:

  • Downregulated SET7 is associated with poor prognosis in colorectal cancer.
  • SET7 functions as a tumor suppressor in colon cancer by modulating HDAC6 activity and acetylated α-tubulin levels.
  • SET7 represents a potential therapeutic target for improving prognosis and preventing metastasis in colon cancer.
Keywords:
Colon cancerSET7/9acetylationdeacetylationhistone deacetylase 6α-tubulin

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