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Updated: Dec 25, 2025

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

20.1K

Inflammation and Vascular Calcification Causing Effects of Oxidized HDL are Attenuated by Adiponectin in Human

Noor Hanisa Harun1, Gabriele Ruth Anisah Froemming2, Hapizah Md Nawawi1,3

  • 1Faculty of Medicine, Universiti Teknologi MARA, Cawangan Selangor, Sungai Buloh Campus, Selangor, Malaysia.

International Journal of Molecular and Cellular Medicine
|March 21, 2020
PubMed

Insights

Oxidized high-density lipoprotein (oxHDL) promotes vascular calcification and inflammation. Adiponectin protects against these detrimental effects by suppressing oxHDL-induced changes in human vascular smooth muscle cells.

Area of Science:

  • Cardiovascular Biology
  • Cellular and Molecular Medicine
  • Biochemistry

Background:

  • The role of oxidized high-density lipoprotein (oxHDL) in vascular calcification is unclear.
  • Adiponectin's protective mechanisms against oxHDL-induced vascular damage require further investigation.

Purpose of the Study:

  • To investigate the effects of oxHDL on inflammation and vascular calcification.
  • To determine if adiponectin can attenuate the detrimental effects of oxHDL.

Main Methods:

  • Human aortic vascular smooth muscle cells (HAoVSMCs) were treated with oxHDL and/or adiponectin.
  • Assays for cell viability, mineralization, and calcification were performed.
  • Protein expression of key inflammatory and osteogenic markers was measured using ELISA kits.

Main Results:

  • Oxidized high-density lipoprotein (oxHDL) induced vascular calcification and increased secretion of IL-6, osterix, WNT-5a, and NF-κβ(p65).
  • Adiponectin suppressed oxHDL-induced vascular calcification, inflammation, and osteogenic marker expression.
  • Adiponectin alone reduced inflammatory cytokines and osteoblastic markers in HAoVSMCs.

Conclusions:

  • Oxidized high-density lipoprotein (oxHDL) plays a significant role in inducing vascular inflammation and calcification.
  • Adiponectin demonstrates a protective effect by attenuating the adverse impacts of oxHDL on vascular cells.

Keywords:
HAoVSMCsadiponectinosteoblastic transdifferentiationoxidized high-density lipoproteinvascular calcification

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