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Cell Seeding Process Experiment and Simulation on Three-Dimensional Polyhedron and Cross-Link Design Scaffolds
Ziyu Liu1, Maryam Tamaddon1, Yingying Gu1
1Division of Surgery and Interventional Science, Royal National Orthopaedic Hospital, University College London, London, United Kingdom.
Frontiers in Bioengineering and Biotechnology
|March 21, 2020
Summary
Optimizing scaffold design is crucial for tissue engineering. This study simulated cell attachment in truncated octahedron and cubic structures, finding simulation aids in predicting cell distribution for better scaffold development and reduced animal testing.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Computational Biology
Background:
- Cell attachment to scaffolds is critical for tissue engineering success.
- Cell seeding efficiency and distribution significantly impact scaffold performance.
- Scaffold design is a key factor in maximizing cell attachment.
Purpose of the Study:
- To investigate optimal scaffold structures for cell attachment.
- To compare cell attachment in truncated octahedron (TO) and cubic scaffold designs.
- To develop and validate a simulation model for predicting cell seeding in 3D scaffolds.
Main Methods:
- Utilized ANSYS Fluent with Volume of Fluid (VOF), Discrete Phase Model (DPM), and Cell Impingement Model (CIM).
- Developed a simulation approach for cell seeding within scaffold structures.
- Validated simulation results with in vitro cell culture assays.
Main Results:
- Both TO and cubic designs exhibited gradual lateral variations in attached cells.
- Simulations accurately modeled cell adhesion in 3D structures.
- Live cells showed limited movement by diffusion; dead cells required external force.
Conclusions:
- The developed simulation model accurately predicts cell distribution in scaffolds.
- This approach aids in optimizing scaffold structures for improved tissue formation.
- The method offers a way to reduce animal testing in scaffold development.
