Streptomyces tailanensis sp. nov., an actinomycete isolated from riverside silt

A novel actinobacterium, designated TRM68348^T, was isolated from the silt collected from the Tailan River in Xinjiang Province, north-west China. The strain was aerobic and Gram-stain-positive. The aerial mycelium was densely straight or tortuous, with a few branches of hyphae and no spores. The whole-cell sugar pattern of strain TRM68348^T consisted of ribose and glucose. The diagnostic diamino was ll-diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannose and an unidentified phospholipid. The predominant menaquinones were MK-9 (H₁₀), MK-9 (H₆) and MK-9 (H₂). The major fatty acids (>5 %) were iso-C_14 : 0, iso-C_15 : 0, anteiso-C_15 : 0, iso-C_16 : 0, C_16 : 0 and summed feature 6. The G+C content of the genomic DNA was 69.93 mol%. Phylogenetic analysis showed that strain TRM68348^T shared 16S rRNA gene sequence similarity of 98.14 % to the closest described species Streptomyces capitiformicae 1H-SSA4^T. Strain TRM68348^T had a relatively low DNA-DNA relatedness value with S. capitiformicae 1H-SSA4^T as determined by calculating the average nucleotide identity value (92.78 %). Strain TRM68348^T could also be differentiated from S. capitiformicae 1H-SSA4^T based on morphological and physiological characteristics. On the basis of the evidence from this polyphasic study, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces tailanensis sp. nov. is proposed. The type strain is TRM68348^T (=CCTCC AA 2018086^T=KCTC 49274^T).