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MicroRNA-29c-3p inhibits osteosarcoma cell proliferation through targeting PIK3R3

H-Z Ma1, J Wang, J Shi

  • 1Department of Orthopedic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. mahuanzhi2006@163.com.

European Review for Medical and Pharmacological Sciences
|March 21, 2020
PubMed
Abstract

Insights

MicroRNA-29c-3p is downregulated in osteosarcoma (OS) and linked to poor prognosis. This microRNA may suppress OS progression by regulating PIK3R3 expression, offering potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs crucial for pathological regulation.
  • MicroRNA-29c-3p is recognized as a tumor suppressor gene in various cancers.
  • The specific role of microRNA-29c-3p in osteosarcoma (OS) progression remained uninvestigated.

Purpose of the Study:

  • To elucidate the function of microRNA-29c-3p in osteosarcoma.
  • To investigate the correlation between microRNA-29c-3p expression and clinical outcomes in OS patients.
  • To explore the underlying molecular mechanism of microRNA-29c-3p in OS progression.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to assess microRNA-29c-3p levels in tumor and adjacent tissues, and OS cell lines.
  • Construction of microRNA-29c-3p knockdown and overexpression models in OS cells.
  • Cell proliferation (CCK-8) and invasion (Transwell) assays to evaluate cellular functions.
  • Western Blot and cell recovery experiments to explore the regulatory mechanism.

Main Results:

  • MicroRNA-29c-3p expression was significantly lower in OS tumor tissues compared to adjacent tissues.
  • Low microRNA-29c-3p expression correlated with increased distant metastasis and poorer overall survival in OS patients.
  • Knockdown of microRNA-29c-3p enhanced OS cell proliferation and invasion, while overexpression inhibited these processes.
  • MicroRNA-29c-3p was found to negatively regulate PIK3R3 expression, with a reciprocal correlation observed in OS tissues.

Conclusions:

  • MicroRNA-29c-3p is downregulated in osteosarcoma and associated with advanced disease and unfavorable prognosis.
  • MicroRNA-29c-3p exerts tumor-suppressive effects in osteosarcoma, potentially by inhibiting PIK3R3 expression.
  • Modulation of microRNA-29c-3p and its target PIK3R3 may represent a novel therapeutic strategy for osteosarcoma.

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