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Updated: Dec 25, 2025

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Published on: July 7, 2023
Contaminated and misidentified cell lines commonly use in cancer research
Siu Tim Cheung1,2, Stephen L Chan3, Kwok Wai Lo4
1Department of Surgery, The Chinese University of Hong Kong, Shatin, Hong Kong.
Abstract:
Cell line authentication is important for credibility concern and scientific reproducibility. Authenticated cancer cell lines retain the properties of the cancers of origin and serve valuable resources for medical research. Experimental results commonly will be validated in more than one cell line to avoid specific cell line effect not generalizable to the disease on the whole. The use of appropriate and verified cell lines would therefore be very important in preclinical studies of translational research, bridging basic research to clinics.
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