Limb blood flow and muscle oxygenation responses during handgrip exercise above vs. below critical force

This study compared the brachial artery blood flow (Q̇_BA) and microvascular oxygen delivery responses during handgrip exercise above vs. below critical force (CF; the isometric analog of critical power). Q̇_BA and microvascular oxygen delivery are important determinants of oxygen utilization and metabolite accumulation during exercise, both of which increase progressively during exercise above CF. However the Q̇_BA and microvascular oxygen delivery responses above vs. below CF remain unknown. We hypothesized that Q̇_BA, deoxygenated-heme (deoxy-[heme]; an estimate of microvascular fractional oxygen extraction), and total-heme concentrations (total-[heme]; an estimate of changes in microvascular hematocrit) would demonstrate physiological maximums above CF despite increases in exercise intensity. Seven men and six women performed 1) a 5-min rhythmic isometric-handgrip maximal-effort test (MET) to determine CF and 2) two constant target-force tests above (severe-intensity; S1 and S2) and two constant target-force tests below (heavy-intensity; H1 and H2) CF. CF was 189.3 ± 16.7 N (29.7 ± 1.6%MVC). At end-exercise, Q̇_BA was greater for tests above CF (S1: 418 ± 147 mL/min; S2: 403 ± 137 mL/min) compared to tests below CF (H1: 287 ± 97 mL/min; H2: 340 ± 116 mL/min; all p < 0.05) but was not different between S1 and S2. Further, end-test Q̇_BA during both tests above CF was not different from Q̇_BA estimated at CF (392 ± 37 mL/min). At end-exercise, deoxy-[heme] was not different between tests above CF (S1: 150 ± 50 μM; S2: 155 ± 57 μM), but was greater during tests above CF compared to tests below CF (H1: 101 ± 24 μM; H2: 111 ± 21 μM; all p < 0.05). At end-exercise, total-[heme] was not different between tests above CF (S1: 404 ± 58 μM; S2: 397 ± 73 μM), but was greater during tests above CF compared to H1 (352 ± 58 μM; p < 0.01) but not H2 (371 ± 57 μM). These data suggest limb blood flow limitations exist and maximal levels of muscle microvascular oxygen delivery and extraction occur during exercise above, but not below, CF.