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Related Experiment Video

Updated: Dec 25, 2025

Isolation of Murine Lymph Node Stromal Cells
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Lymph node stromal CCL2 limits antibody responses.

Dragos C Dasoveanu1,2, Hyeung Ju Park3, Catherine L Ly3

  • 1Physiology Biophysics and Systems Biology, Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA.

Science Immunology
|March 22, 2020
PubMed
Summary
This summary is machine-generated.

Lymph node stromal cells expressing CCL2 limit antibody-forming cell survival by influencing monocyte function. This discovery reveals a regulatory role for stromal cells in controlling immune responses and suggests therapeutic targets for autoimmunity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Lymphatic System Research

Background:

  • Nonhematopoietic stromal cells, including fibroblastic reticular cells (FRCs), support antibody-forming cell (AFC) survival in lymph nodes.
  • The regulatory role of the stromal compartment in AFC accumulation has not been fully understood.

Purpose of the Study:

  • To investigate the regulatory function of lymph node stromal cells in AFC accumulation.
  • To elucidate the mechanisms by which stromal cells influence AFC survival and numbers.

Main Methods:

  • Analysis of chemokine ligand 2 (CCL2) expression in lymph node stromal cells.
  • Lymph node transplantation experiments to assess the role of CCL2 in AFC survival.
  • Monocyte depletion and transfer studies to evaluate their function in AFC responses.
  • Assessment of reactive oxygen species (ROS) production by monocytes.

Main Results:

  • Stromal cells expressing CCL2 were found to limit AFC survival.
  • CCL2 deficiency in FRCs and lymphatic endothelial cells increased AFC survival.
  • Monocytes expressing CCR2 were crucial for regulating AFC accumulation and ROS production.
  • Stromal CCL2 modulated monocyte accumulation and ROS production, partly via vascular permeability.

Conclusions:

  • The lymph node stromal compartment plays a regulatory role in AFC survival by influencing monocyte accumulation and function.
  • Inflammation-induced vascular activity may tune the lymph node microenvironment.
  • Understanding stromal-mediated AFC regulation could offer strategies for controlling antibody-mediated autoimmunity.