Related Experiment Video
Updated: Dec 25, 2025
05:34
Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
2.5K
Campylobacter upsaliensis isolated from a giant hepatic cyst
Yasuo Ohkoshi1, Toyotaka Sato2, Hiromi Murabayashi3
1Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan; Department of Clinical Laboratory, NTT Medical Center Sapporo, Sapporo, Japan.
Summary
This study reports the first isolation of Campylobacter upsaliensis from a giant hepatic cyst. Advanced whole genome sequencing was crucial for identification when standard methods failed.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Campylobacter upsaliensis is an enteropathogenic bacterium primarily found in animals.
- It can cause human infections like enteritis and bacteremia, though rarely isolated.
Observation:
- This is the first reported case of Campylobacter upsaliensis isolated from a giant hepatic cyst.
- The bacterium was not culturable on standard selective media due to high cefoperazone concentration.
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry also failed for identification.
Findings:
- Whole genome sequencing, including multilocus sequence typing, successfully identified the bacterium as Campylobacter upsaliensis.
- This highlights the limitations of conventional methods for certain bacterial identifications.
Implications:
- This case expands the known clinical manifestations of Campylobacter upsaliensis infections.
- Whole genome sequencing offers a powerful alternative for identifying difficult-to-culture or misidentified bacteria.
- Further research may be needed to understand the prevalence and pathogenesis of C. upsaliensis in hepatic cysts.
