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Updated: Dec 25, 2025
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
A study on bone tissue engineering: Injectable chitosan-g-stearic acid putty
Volkan Yalman1, Ekin Çelik2, Ömer Arslan3
1Department of Molecular Biology and Genetics, Yıldız Technical University, Istanbul, 34349, Turkey.
Background:
Bioengineering products can help bone tissue regeneration.
Objective:
There is an ongoing research for more effective biomaterials in bone regeneration. Chitosan (Ch) grafted stearic acid (Ch-g-Sa) polymer was synthesized and its usability as a putty was evaluated in this study.
Methods:
The chemical structure of Ch-g-Sa polymer was investigated using Proton nuclear magnetic resonance (H-NMR) and Fourier-transformed infrared spectroscopy-attenuated total reflectance (FTIR-ATR). Thermal properties of Ch-g-Sa polymer were determined by thermal gravimetric analysis (TGA). Putties containing nano-hydroxyapatite were prepared and in-vitro degradation properties and viscosity of the putties were determined.
Results:
The cytotoxicity, oxidation effect and osteogenic potential of the putties were investigated on MC3T3 cells while the inflammatory effect of the putties was studied on THP-1 cells. For the determination of the osteogenic effect of the putties, ALP and RUNX2 gene expression of MC3T3 cells were studied.
Conclusion:
Ch-g-Sa/HA putties are promising biomaterials for bone tissue regeneration.
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