Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K
Epigenetic Regulation01:37

Epigenetic Regulation

3.6K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nucleosome wrapping energy in CpG islands and the role of epigenetic base modifications.

eLife·2025
Same author

Mapping chromatin structure at base-pair resolution unveils a unified model of cis-regulatory element interactions.

Cell·2025
Same author

Chromatin profiling identifies putative dual roles for H3K27me3 in regulating cell type-specific genes and transposable elements in choanoflagellates.

Nature communications·2025
Same author

Discovery and Characterization of Uracil Derivatives Targeting the Set-and-Ring Domain of UHRF1.

Journal of chemical information and modeling·2025
Same author

Platelets sequester extracellular DNA, capturing tumor-derived and free fetal DNA.

Science (New York, N.Y.)·2025
Same author

MGA directly recruits SETDB1/ATF7IP for histone H3K9me3 mark on meiosis-related genes in mouse embryonic stem cells.

iScience·2025

Related Experiment Video

Updated: Dec 25, 2025

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K

ATACing DNA Methylation during Differentiation.

Skirmantas Kriaucionis1, Robert J Klose2

  • 1Ludwig Cancer Research Oxford, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK.

Molecular Cell
|March 24, 2020
PubMed
Summary

DNA methylation removal is not required for chromatin accessibility at active gene regulatory elements during cellular differentiation. Researchers developed a new technology, ATAC-Me, to study this process.

More Related Videos

ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

5.1K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.4K

Related Experiment Videos

Last Updated: Dec 25, 2025

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K
ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

5.1K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.4K

Area of Science:

  • Genomics
  • Epigenetics
  • Molecular Biology

Background:

  • Distal regulatory elements are crucial for controlling gene expression during cellular differentiation.
  • Understanding the mechanisms of chromatin accessibility is key to deciphering gene regulation.

Purpose of the Study:

  • To investigate the role of DNA methylation in chromatin accessibility at active gene regulatory elements during cellular differentiation.
  • To develop and apply a novel technology for studying these processes.

Main Methods:

  • Development of a new technology named ATAC-Me.
  • Application of ATAC-Me to analyze chromatin accessibility and DNA methylation dynamics during differentiation.

Main Results:

  • The study found that removal of DNA methylation is not a prerequisite for the establishment of accessible chromatin.
  • Accessible chromatin can form at active gene regulatory elements independently of DNA demethylation during differentiation.

Conclusions:

  • The findings challenge the established model where DNA demethylation is considered essential for chromatin opening.
  • This suggests alternative or parallel pathways for regulating chromatin accessibility during cellular differentiation.