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Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
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DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli.

Gaser N Abdelrasoul1, Afreen Anwar2, Scott MacKay1

  • 1Electrical, and Computer Engineering Department, University of Alberta, Edmonton, AB, Canada.

Analytica Chimica Acta
|March 24, 2020
PubMed
Summary
This summary is machine-generated.

We developed a portable, inexpensive impedance biosensor using Interdigitated Electrode (IDE) arrays to detect Escherichia coli (E. coli). This novel sensor achieves a low limit of detection, offering a rapid alternative to traditional methods for pathogen detection.

Keywords:
Aptamer-based sensorE. coliElectrochemical biosensorWater quality monitoring and environmental sensor

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Area of Science:

  • Biosensor Technology
  • Electrochemical Sensing
  • Microbial Detection

Background:

  • Conventional methods for pathogenic bacteria detection (e.g., ELISA, PCR) are costly, time-consuming, and require expert operation.
  • There is a critical need for real-time, portable, and inexpensive sensors for pathogenic bacteria.
  • Impedance-based biosensors offer a promising label-free detection approach.

Purpose of the Study:

  • To develop and demonstrate a portable, inexpensive impedance-based biosensor for detecting Escherichia coli (E. coli).
  • To investigate factors influencing the sensitivity and performance of the biosensor, including substrate material, aptamer concentration, and surface chemistry.
  • To establish the feasibility of using aptamer-functionalized Interdigitated Electrode (IDE) arrays for microbial detection.

Main Methods:

  • Fabrication of Interdigitated Electrode (IDE) arrays on various substrate materials.
  • Functionalization of IDE surfaces with an E. coli outer membrane protein (OMP) Ag1 Aptamer for specific binding.
  • Electrochemical impedance spectroscopy was used to detect E. coli in the concentration range of 25-1000 cfu mL⁻¹.
  • Surface characterization using Field Emission Scanning Electron Microscopy (FESEM), Optical Microscopy, and Atomic Force Microscopy (AFM).

Main Results:

  • The developed impedance-based biosensor successfully detected E. coli with an analytical sensitivity of approximately 1.8 Ohm/cfu.
  • A limit of detection (LOD) of 9 cfu mL⁻¹ was achieved by optimizing the sensor's surface chemistry, substrate material, and aptamer concentration.
  • The molecular composition of the self-assembled monolayer (SAM) significantly impacted sensor sensitivity.

Conclusions:

  • The portable, inexpensive impedance-based biosensor demonstrates high sensitivity and a low LOD for E. coli detection.
  • This aptamer-functionalized IDE platform offers a viable alternative to conventional methods for rapid microbial analysis.
  • The sensor platform is adaptable for detecting other microorganisms and chemicals relevant to environmental monitoring and public health.