Development of a cysteine sensor based on the peroxidase-like activity of AgNPs@ Fe₃O₄ core-shell nanostructures

In this study, a facile one step solvo-thermal procedure has been employed in generating magnetite-silver core-shell nanocomposites (AgNPs@ Fe₃O₄) with superior peroxidase-like catalytic property than bare magnetic nanoparticles (Fe₃O₄). The composites were characterized using different techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and surface-enhanced infrared absorption spectroscopy (SEIRA). In the presence of hydrogen peroxide, the synthesized composites were able to oxidize the colorless o-phenylenediamine (OPD) to a yellow colour 2, 3-diaminophenazine (DAP) with a better peroxidase-like activity than Fe₃O₄ alone. The obtained K_m value of AgNPs@ Fe₃O₄ with H₂O₂ and OPD substrates are 28.0 mM and 2.91 mM respectively. These are substantially lower than previously reported values and indicate the strong binding affinity of the substrates towards AgNPs@ Fe₃O₄ nanocomposites. Based on the obstruction activity of cysteine on the peroxidase-like catalytic property of the nanocomposites, a sensor was developed for detection of cystein with a limit of detection as low as 87 nM and a wider range of linearity. The sensor also exhibited excellent selectivity against potentially interfering molecules.