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Updated: Dec 25, 2025

Shotgun Lipidomics of Rodent Tissues
Published on: November 18, 2022
Very long-term stability of lipid biomarkers in human serum
Vladimira Muzakova1, Piet K Beekhof2, Eugène H J M Jansen2
1Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
Abstract:
The assessment of the stability of biomarkers is very important for epidemiological studies. In this study, the stability of five lipid parameters (total cholesterol, triglycerides, HDL- and LDL-cholesterol and free fatty acids) has been tested in 16 human serum samples after storage at -80 °C up at time points 0, 1, 8 and 13 y. The majority of the lipid biomarkers were stable during storage conditions, except for cholesterol. The correlations between the samples were very good at time points. Therefore, long-term storage of human serum samples allows lipid biomarker determination, provided that the samples are stored at -80 °C.
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