Related Experiment Video
Updated: Dec 25, 2025
11:12
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
8.3K
Differences in Endothelin B Receptor Isoforms Expression and Function in Breast Cancer Cells
Meena Halaka1, Zuhaila A Hired1, Grace E Rutledge1
1Department of Biology, College of Liberal Arts and Sciences, Lipscomb University, 1 University Park Drive, Nashville, TN 37204, USA.
Journal of Cancer
|March 24, 2020
Summary
This study reveals that specific endothelin B receptor (EDNRB) gene isoforms play a crucial role in breast cancer progression and patient survival. Understanding these EDNRB isoforms offers new insights into breast cancer development.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Endothelins and their receptors are known for vascular regulation.
- Their presence in epithelial cells and tumors suggests a role in cancer progression.
Purpose of the Study:
- To investigate the expression of endothelin B receptor (EDNRB) gene isoforms in breast cancer.
- To determine the role of EDNRB isoforms in breast cancer cell invasiveness, signaling, and patient survival.
Main Methods:
- Assessed mRNA expression of major EDNRB isoforms in normal breast cells and cancer cell lines.
- Performed gene knockdown experiments to study the effect on invasiveness.
- Analyzed isoform-specific regulation of cell invasion, signaling, and patient survival.
Main Results:
- Found differential mRNA and protein expression of EDNRB isoforms in normal versus cancer breast cells.
- EDNRB gene knockdown altered breast cancer cell invasiveness towards endothelin 3 (ET3).
- Observed EDNRB isoform-specific regulation of breast cancer cell invasion, signaling, and patient survival.
Conclusions:
- The endothelin B receptor (EDNRB) is important in breast cancer.
- This study is the first to elucidate the differential expression and roles of specific EDNRB isoforms in breast cancer.
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
3.2K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.2K
01:26Adrenergic Receptors: β Subtype
3.3K
β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
3.3K
01:15Receptor Downregulation in MVBs
2.7K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.7K
01:18Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists
363
Endothelins (ETs) are potent vasoactive peptides critical in the human body's various physiological and pathological processes. One of the most promising therapeutic strategies for treating pulmonary arterial hypertension (PAH) involves counteracting the effects of these endothelins using a class of drugs known as endothelin receptor antagonists.
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme...
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme...
363
02:38Mitogens and the Cell Cycle
7.6K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.6K
01:27Transducer Mechanism: Enzyme-Linked Receptors
3.7K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
3.7K