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Updated: Dec 25, 2025

Monitoring Protein Adsorption with Solid-state Nanopores
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Measuring Enzymatic Activities with Nanopores.

Yingying Sheng1, Shouwen Zhang2, Lei Liu3

  • 1Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Chembiochem : a European Journal of Chemical Biology
|March 24, 2020
PubMed
Summary

Nanopore enzymology uses nanopore technology to study enzymes, measuring their activity and products. This field offers new ways to analyze enzymes for applications like DNA sequencing.

Keywords:
enzymatic activitykineticsnanoporesquantitative measurementssingle-molecule studies

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Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Nanopores are single-molecule sensing tools that emerged in the mid-1990s.
  • They detect various analytes like ions, organic molecules, DNA/RNA, and proteins.
  • Recent advancements focus on applying nanopore technology to study enzymes.

Purpose of the Study:

  • To review recent developments in nanopore enzymology.
  • To categorize current research into three main areas.
  • To discuss future challenges and opportunities in the field.

Main Methods:

  • Measuring enzymatic activities using nanopore-enzyme hybrids.
  • Detecting enzymatic activities by sensing their catalytic products via nanopores.
  • Utilizing enzymes in conjunction with nanopores for DNA sequencing and molecule translocation.

Main Results:

  • Demonstrated the versatility of nanopores for enzyme activity measurement.
  • Highlighted three distinct approaches within nanopore enzymology.
  • Showcased enzyme applications in DNA sequencing and translocation.

Conclusions:

  • Nanopore enzymology is a rapidly advancing field with significant potential.
  • Further research is needed to overcome current challenges.
  • The technology offers promising avenues for biochemical analysis and molecular manipulation.