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Updated: Dec 25, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
Published on: October 18, 2022
Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
Nicole D Marino1, Rafael Pinilla-Redondo1,2,3, Bálint Csörgő1,4
1Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
Abstract:
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.
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