Related Experiment Video

Updated: Dec 25, 2025

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
07:25

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Published on: December 17, 2019

8.6K

Macrophages represent the major pool of IL-7Rα expressing cells in patients with myocarditis

Natalia Kubin1, Manfred Richter1, Bedriye Sen-Hild2

  • 1Department of Cardiac Surgery, Kerckhoff-Clinic, Benekestrasse 2-8, Bad Nauheim 61231, Germany.

Cytokine
|March 24, 2020
PubMed

Insights

Myocarditis involves cardiac interleukin-7 receptor-alpha (IL-7Rα) expressing macrophages, not just T cells. These IL-7Rα+ macrophages may influence heart failure development.

Area of Science:

  • Immunology
  • Cardiology
  • Molecular Biology

Background:

  • Myocarditis, often infection-induced, involves inflammatory responses and can lead to heart failure.
  • Cytokine and chemokine receptors play a role in myocarditis pathogenesis.
  • Interleukin-7 (IL-7) signaling is crucial for lymphocyte development and survival.

Purpose of the Study:

  • To identify specific immune cell receptor mediators involved in myocarditis.
  • To investigate the role of interleukin-7 receptor-alpha (IL-7Rα) in cardiac inflammation during myocarditis.

Main Methods:

  • Confocal microscopy was used to screen various receptors in cardiac tissue from myocarditis patients.
  • Immunohistochemical analysis identified IL-7Rα expressing cells and characterized them using leukocyte, B cell, T cell, and macrophage markers.

Main Results:

  • Cardiac cells expressing IL-7Rα were identified in myocarditis patients, with immune cells being scarce in controls.
  • Macrophages (CD68+) and T cells (CD3+) were the main inflammatory populations in myocarditis.
  • A high percentage of macrophages (90%) expressed IL-7Rα, while fewer T cells (less than 20%) did.

Conclusions:

  • The study identified IL-7Rα+ macrophages as a significant component of cardiac inflammation in myocarditis.
  • IL-7Rα+ macrophages may orchestrate cardiac inflammation and potentially regulate lymphocyte responses, influencing myocarditis progression and heart failure development.
Keywords:
Immune cellsInflammationInterleukin-7 receptor-αMacrophagesMyocarditis

More Related Videos

Isolation and Identification of Extravascular Immune Cells of the Heart08:24

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

13.8K
Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node08:43

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Published on: May 7, 2021

5.0K

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.0K
Myocarditis I: Introduction01:21

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
283
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
7.7K
Myocarditis II: Clinical Features and Diagnostic Tests01:27

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
192
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.4K