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Related Experiment Video

Updated: Dec 25, 2025

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

An Effective and Efficient Testing Protocol for Diagnosing Iron-deficiency Anemia Preoperatively.

Obianuju Okocha¹, Hardik Dand, Michael J Avram

¹From the Department of Anesthesiology, Northwestern University Feinberg School of Medicine, Chicago, Illinois (O.O., M.J.A., B.J.S.) Midwestern University, Downers Grove, Illinois (H.D.).

|March 25, 2020

Summary

A new preprocedure anemia evaluation protocol significantly improves the diagnosis of iron-deficiency anemia (IDA) in patients before surgery. This efficient, single-visit approach identifies more IDA cases than traditional methods, improving patient care.

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Area of Science:

Perioperative Medicine
Hematology
Clinical Diagnostics

Background:

Iron-deficiency anemia (IDA) is a prevalent perioperative condition associated with increased morbidity and mortality.
Timely diagnosis and treatment of IDA are crucial for improving surgical outcomes.

Purpose of the Study:

To evaluate a novel preprocedure anemia evaluation protocol for diagnosing iron-deficiency anemia (IDA).
To compare the diagnostic yield of the new protocol against traditional screening methods.

Main Methods:

A retrospective cohort study compared patients before and after implementing a new anemia evaluation protocol.
The protocol involved a complete blood count with reflex anemia testing, completed in a single visit.
Iron-deficiency anemia was defined by specific hemoglobin, serum ferritin, and transferrin saturation levels.

Main Results:

The traditional complete blood count (CBC) diagnosed 1.2% of patients with IDA preoperatively.
The new protocol diagnosed 26.1% of patients with IDA, a significantly higher proportion.
The preprocedure anemia evaluation demonstrated a substantially greater ability to identify IDA compared to CBC alone.

Conclusions:

The preprocedure anemia evaluation protocol enhances the identification of iron-deficiency anemia in the preoperative setting.
This protocol is more effective and efficient, enabling comprehensive anemia assessment within a single patient visit.