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Cusp-artifacts in high order superresolution optical fluctuation imaging.

Xiyu Yi1,2,3,4, Shimon Weiss1,5,6,7,8

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles 90095, USA.

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|March 25, 2020
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Summary
This summary is machine-generated.

High-order superresolution optical fluctuation imaging (SOFI) suffers from "cusp artifacts," limiting resolution. This study identifies these artifacts and their impact, offering guidelines for improved SOFI imaging and probe development.

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Area of Science:

  • Biophysics
  • Optical Imaging
  • Super-resolution Microscopy

Background:

  • Superresolution optical fluctuation imaging (SOFI) is a widely adopted, cost-effective super-resolution microscopy technique.
  • Despite its popularity, the theoretical resolution enhancements of high-order SOFI have not been fully realized.

Purpose of the Study:

  • To identify and characterize artifacts limiting high-order SOFI resolution.
  • To provide guidelines for improving SOFI data acquisition and fluorescence probe selection.

Main Methods:

  • Analysis of high-order cumulants, odd-order moments, and balanced-cumulants (bSOFI) in the presence of artifacts.
  • Investigated the impact of

Main Results:

  • Identified

Conclusions:

  • The identified