Osteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation
Jianqiao Zheng1,2,3,4,5, Xinyue Zhang1,2,3,4,5, Yaopeng Zhang1,2,3,4,5
1Center of Digital Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Abstract:
This study examines the osteogenic effect of femtosecond laser bone ablation on bone mesenchymal stromal cells (BMSCs). Three-week old Sprague-Dawley (SD) rats were selected for experiments. Right tibias were ablated by a 10-W femtosecond laser (treated group), whereas left tibias were not subjected to laser ablation (control group). After ablation, BMSCs of both tibias were cultured and purified separately. Cell proliferation was then analyzed, as well as the expressions of RNA and several proteins (alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN)). The results suggest that femtosecond laser ablation promotes the differentiation of BMSCs and up-regulates the expression of ALP, RUNX2, and OCN, without affecting BMSC proliferation.
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