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Bioprinting for Skin
Cristina Quílez1, Gonzalo de Aranda Izuzquiza1, Marta García1,2,3
1Department of Bioengineering & Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), Madrid, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2020
Summary
Researchers developed a rapid 3D bioprinting method for human skin. This innovative technique produces skin tissue comparable to native human skin for potential clinical applications.
Area of Science:
- Biotechnology
- Tissue Engineering
- Regenerative Medicine
Background:
- Current methods for producing skin grafts are time-consuming and labor-intensive.
- There is a need for faster, scalable methods to generate functional human skin tissue.
Purpose of the Study:
- To develop and evaluate an extrusion-based 3D bioprinting method for creating human bilayered skin.
- To assess the structural and functional similarity of the printed skin to native human skin.
Main Methods:
- Utilized bioinks composed of human plasma, fibroblasts, and keratinocytes.
- Employed an extrusion-based bioprinting system to generate 100 cm² of skin in under 35 minutes.
- Conducted histological and immunohistochemical analyses on in vitro 3D cultures and transplanted skin in immunodeficient mice.
Main Results:
- Successfully printed human bilayered skin with a high yield and speed.
- Demonstrated that the printed skin exhibits structural characteristics similar to normal human skin.
- The printed skin was indistinguishable from manually produced bilayered dermo-epidermal equivalents used clinically.
Conclusions:
- The described extrusion-based bioprinting method offers a rapid and efficient approach for generating functional human skin.
- The printed skin holds significant potential for clinical applications in skin regeneration and wound healing.
- This technology represents a promising advancement in the field of tissue engineering for skin replacement.
