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Circular RNA circMTO1 Suppresses RCC Cancer Cell Progression via miR9/LMX1A Axis

Kecheng Li1, Cheng-Liang Wan2, Yan Guo3

  • 1Department of clinical laboratory, Liuzhou People's Hospital, Liuzhou, Guangxi, China.

Technology in Cancer Research & Treatment
|March 25, 2020
PubMed

Insights

Circular RNAs like circMTO1 suppress renal cell carcinoma progression by sponging microRNAs. This circMTO1/miR9/LMX1A pathway offers a potential therapeutic target for kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is a prevalent adult kidney cancer.
  • Circular RNAs (circRNAs) are emerging regulators in cancer, often acting as microRNA sponges.
  • The role of circRNAs in RCC pathogenesis is largely unknown.

Purpose of the Study:

  • To investigate the function of a novel, highly expressed circRNA, circMTO1, in renal cell carcinoma.
  • To elucidate the molecular mechanism underlying circMTO1's role in RCC progression.

Main Methods:

  • Expression analysis of circMTO1 in RCC cell lines.
  • Functional assays (proliferation, metastasis) upon circMTO1 overexpression or silencing.
  • MicroRNA sponge activity assessment and target gene validation (miR9, LMX1A).

Main Results:

  • circMTO1 overexpression suppressed proliferation and metastasis in A497 and 786-O cells.
  • circMTO1 silencing promoted tumor progression in SN12C and OS-RC-2 cells.
  • circMTO1 functions as a sponge for miR9 and miR223, inhibiting their activity and consequently regulating LMX1A expression.

Conclusions:

  • circMTO1 acts as a tumor suppressor in renal cell carcinoma.
  • The circMTO1/miR9/LMX1A axis is a key pathway in RCC progression.
  • circMTO1 represents a potential therapeutic target for renal cell carcinoma treatment.
Keywords:
LMX1AcircMTO1circular RNAmiR9renal cell carcinoma

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