Insilico study of genes involved in Congenital Hypothyroidism
Maria Javed1, Ammara Masood1, Iqra Baig1
1Department of Biotechnology, University of Central Punjab Lahore, Pakistan.
Objective:
To study the orthologs of the five genes of congenital hypothyroidism NIS, PAX8, DUOX2, FOXE1, NKX2-1 that are involved in the development of the thyroid gland.
Methods:
The study was conducted at INMOL Cancer Hospital, Lahore in September 2017 and comprised of finding gene orthologs, phylogenetic tree and domains of NIS, PAX8, DUOX2, FOXE1, NKX2-1 which were studied using different bioinformatics tools, including FASTA, BLAST, ENSEMBL, UniProt, MultiAlin, to find out the important domains involved in the mutations of these genes.
Results:
Genes showed consensus sequence / motifs involved in congenital hypothyroidism. Phylogenetic results showed that these genes shared some common motifs. Phylogenetic trees revealed sub-clusters with high protein homology.
Conclusions:
Genes involved in congenital hypothyroidism were found to have a consensus sequence motifs.
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