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Updated: Dec 25, 2025

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

AAA+ proteins.

Thiago V Seraphim¹, Walid A Houry²

¹Department of Biochemistry, University of Toronto, Toronto, ON M5G 1M1, Canada.

Current Biology : CB

|March 26, 2020

Summary

This summary is machine-generated.

This Primer reviews AAA+ proteins, focusing on their diverse structures and functions. It summarizes molecular mechanisms that drive these essential protein activities.

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Area of Science:

Biochemistry
Molecular Biology
Structural Biology

Background:

AAA+ proteins represent a large and functionally diverse superfamily.
These proteins are involved in numerous cellular processes, including DNA replication, protein degradation, and membrane fusion.

Purpose of the Study:

To provide an overview of the structural characteristics of AAA+ proteins.
To summarize the known functional diversity within the AAA+ protein family.
To elucidate the molecular mechanisms underlying AAA+ protein functions.

Main Methods:

Review of existing literature on AAA+ proteins.
Analysis of structural data from various AAA+ proteins.
Synthesis of current knowledge on AAA+ protein mechanisms.

Main Results:

AAA+ proteins share a conserved AAA+ module responsible for ATP hydrolysis.
Structural variations in AAA+ proteins contribute to their diverse functions.
Mechanistic insights reveal common principles of ATP-dependent molecular motion.

Conclusions:

AAA+ proteins are versatile molecular machines with critical cellular roles.
Understanding their structure-function-mechanism relationships is key to deciphering cellular processes.
Further research into AAA+ protein mechanisms will uncover new therapeutic targets.