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The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation.

Patricia A A Buranello¹, Valéria C Barbosa-Lorenzi¹, Marcelo R Pinto¹

¹Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

|March 26, 2020

Summary

This summary is machine-generated.

Artocarpus heterophyllus lectin ArtinM activates mast cells and stimulates proliferation at low concentrations. This immune modulation may offer therapeutic benefits without triggering allergic reactions.

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Area of Science:

Immunology
Cell Biology

Background:

Mast cells are key players in allergic and inflammatory responses.
Mast cell activation releases mediators crucial for immune functions.
ArtinM, a lectin from jackfruit, binds D-mannose and can induce mast cell degranulation.

Purpose of the Study:

To investigate the role of ArtinM in mast cell activation and proliferation.
To explore ArtinM's effects on signaling pathways and cytokine release.
To assess ArtinM's potential therapeutic applications in immune modulation.

Main Methods:

Utilized the rat mast cell line RBL-2H3.
Administered varying concentrations of ArtinM.
Measured IL-6 release, protein tyrosine phosphorylation, NF-kB and NFAT activation, and cell cycle progression.

Main Results:

Low concentration ArtinM (0.25μg/mL) induced mast cell activation and IL-6 release without degranulation.
ArtinM stimulated protein tyrosine phosphorylation and NF-kB activation.
ArtinM promoted cell cycle progression and mast cell proliferation.

Conclusions:

ArtinM modulates mast cell activity, including proliferation and IL-6 release, at low concentrations.
These effects occur without inducing degranulation, suggesting potential therapeutic use.
ArtinM shows promise for immune modulation and therapeutic applications by influencing mast cell behavior.