Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Column-based separation of erythrocytes using aqueous polymeric two-phase systems.

D R Skuse1, D E Brooks

  • 1Department of Chemistry, University of British Columbia, Vancouver, Canada.

Journal of Chromatography
|November 18, 1988
PubMed
Summary

A new column-based cell separation technique using dextran-polyethylene glycol (PEG) systems significantly improves erythrocyte separation purity. This method achieves over 98% purity, outperforming traditional single-tube separations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Equine ulcerative keratitis with furrow: A review of the outcomes of 72 cases from 1987 to 2015.

Equine veterinary journal·2019
Same author

Progesterone binding nano-carriers based on hydrophobically modified hyperbranched polyglycerols.

Nanoscale·2016
Same author

Effect of frailty on short- and mid-term outcomes in vascular surgical patients.

The British journal of surgery·2015
Same author

Visual outcome after corneal transplantation for corneal perforation and iris prolapse in 37 horses: 1998-2010.

Equine veterinary journal. Supplement·2013
Same author

Development and in vitro characterization of paclitaxel and docetaxel loaded into hydrophobically derivatized hyperbranched polyglycerols.

International journal of pharmaceutics·2010
Same author

Targeted lamellar keratoplasty in the horse: a paradigm shift in equine corneal transplantation.

Equine veterinary journal. Supplement·2010

Area of Science:

  • Biochemistry
  • Separation Science
  • Chromatography

Background:

  • Aqueous polymeric two-phase systems are established for cell separation.
  • Multi-step counter-current distribution is used for cells with similar partition coefficients.

Purpose of the Study:

  • To develop an alternative column-based cell separation technique.
  • To improve separation efficiency for cells with similar partition coefficients.

Main Methods:

  • Immobilization of the dextran-rich phase of a dextran-polyethylene glycol (PEG) system onto chromatography beads.
  • Using the PEG-rich phase as the eluent in a column setup.
  • Separation of erythrocytes from different species using an affinity ligand.

Main Results:

Related Experiment Videos

  • A critical affinity ligand concentration was identified for cell elution.
  • Cell recovery was proportional to affinity ligand concentration.
  • A 1:1 mixture of dog and human erythrocytes was separated to >98% purity, significantly exceeding the 84% purity from optimized single-tube separation.

Conclusions:

  • The column-based technique offers superior separation efficiency compared to single-tube methods.
  • This method provides a viable alternative for separating cells with similar partition coefficients.
  • The observed elution behavior suggests a mechanism distinct from multi-step partitioning.