Phycoremediation potential of microalgae species for ethidium bromide removal from aqueous media

Ethidium Bromide (EtBr) is an organic compound used in molecular biology investigations. EtBr ability of intercalating in the DNA molecule makes it a toxic substance. The objective was to evaluate the phycoremediation potentials of Chlorella vulgaris, Desmodesmus subspicatus and Raphidocelis subcapitata tested separately and in a mixture (Mix) for EtBr removal from the aqueous medium. Experiments were conducted using an initial algae biomass of 10⁶ cell/mL, exposed to 500 µg/L of EtBr. The removal efficiency (µg EtBr L^-1) after 3 h in each treatment were: Mix (72.8 µg.L^-1) >D. subspicatus (48.4 µg.L^-1) >R. subcapitata (24.6 µg.L^-1) >C. vulgaris (19.9 µg.L^-1). However, when EtBr mass reduction per microalgae density is considered (ng.algae^-1), the efficiency ranking changes to: D. subspicatus (1.9 × 10^-5ng.algae^-1) >C. vulgaris (1.4 × 10^-5ng.algae^-1) >Mix (9.8 × 10^-6ng.algae^-1) >R. subcapitata (2.8 × 10^-6ng.algae^-1). The results suggest that initial algal population density is a determinant factor for efficient EtBr removal by microalgae species in short term treatments. In order to obtain 100% of EtBr removal, it should be necessary 10¹⁰, 10¹⁰ and 10¹¹ algae.mL^-1 of C. vulgaris, D. subspicatus and R. subcapitata, respectively. The results strongly suggest phycoremediation can be explored as an alternative method for EtBr removal.