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Updated: Dec 25, 2025
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
Comparative profiling of exosomal miRNAs in human adult peripheral and umbilical cord blood plasma by deep sequencing
Sirui Huang1, Zhenlin Tang1, Yuheng Wang1
1School of Life Science & Biopharmacology, Guangdong Pharmaceutical University, Number 280 Outer Ring East Road, Higher Education Mega Center, Guangzhou, Guangdong 510006,PR China.
Abstract:
Aim: To assess differential expression profiles of miRNAs in exosomes derived from human peripheral blood (PB) and umbilical cord blood (UCB). Materials & methods: Small RNA sequencing was performed to characterize the miRNA expression in plasma exosomes processed from UCB of five healthy newborns and PB of five normal adult volunteers, and differentially expressed miRNAs were further analyzed. Results: A total of 65 exosomal miRNAs, including 46 upregulated and 19 downregulated, showed differential expression between UCB and PB. Target genes of these miRNAs were mainly enriched in signaling pathways associated with pregnancy, cancers, cell mobility and nervous system. Conclusion: Exosomal miRNAs may have essential roles in the biological functions of UCB, suggesting the therapeutic and biomarker potentials of exosomes in UCB.
