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RYBP inhibits esophageal squamous cell carcinoma proliferation through downregulating CDC6 and CDC45 in G1-S phase

Yue Ke1, Wei Guo1, Shan Huang1

  • 1Department of Radiation Oncology, Second Affiliated Hospital, Xi'an Jiaotong University, 710004 Xi'an, China.

Life Sciences
|March 27, 2020
PubMed
Abstract

Insights

RING1 and YY1-binding protein (RYBP) suppresses esophageal squamous cell carcinoma (ESCC) growth by downregulating cell cycle genes CDC6 and CDC45. This epigenetic regulator

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Molecular Oncology

Background:

  • RING1 and YY1-binding protein (RYBP) is an epigenetic regulator with known roles in embryonic development.
  • RYBP's anti-tumor effects have been observed in various cancers, but its specific role in esophageal squamous cell carcinoma (ESCC) remains unclear.

Purpose of the Study:

  • To investigate the biological function of RYBP in ESCC.
  • To elucidate the molecular mechanisms underlying RYBP's role in ESCC progression.

Main Methods:

  • Immunohistochemistry on ESCC tissue microarrays to assess RYBP expression.
  • In vitro assays (CCK8, colony formation, flow cytometry) to evaluate cell proliferation and cell cycle.
  • Transcriptome arrays, qRT-PCR, and Western blot to analyze gene expression.
  • In vivo studies using nude mice to assess RYBP's effect on tumor growth.

Main Results:

  • RYBP expression was found to be downregulated in ESCC tissues compared to normal adjacent tissues.
  • Higher RYBP expression correlated with better patient outcomes in ESCC.
  • Overexpression of RYBP inhibited ESCC cell proliferation and tumor growth both in vitro and in vivo.
  • RYBP was shown to decrease the expression of cell cycle-related genes, specifically CDC6 and CDC45, which are critical for DNA replication and G1-S phase transition.

Conclusions:

  • RYBP acts as a tumor suppressor in ESCC.
  • RYBP inhibits ESCC proliferation by downregulating CDC6 and CDC45, thereby impeding the G1-S cell cycle transition.
Keywords:
CDC45CDC6Cell cycleEsophageal squamous cell carcinomaRYBP

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