Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.4K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.4K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

6.3K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.3K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.9K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.9K
Cell Migration01:09

Cell Migration

18.3K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.3K
Cell Migration01:19

Cell Migration

6.2K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.2K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.3K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From biting to engulfment: curvature-actin coupling controls phagocytosis of soft, deformable targets.

bioRxiv : the preprint server for biology·2026
Same author

Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation.

PLoS computational biology·2026
Same author

Trade-off between branching and polarity controls decision-making during cell migration.

Science advances·2026
Same author

Coupling anisotropic curvature and nematic order: mechanisms of membrane shape remodeling.

Soft matter·2025
Same author

Integrated Ising model with global inhibition for decision-making.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation.

The Journal of cell biology·2025
Same journal

RETRACTED: Bakshi et al. Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. <i>Cells</i> 2022, <i>11</i>, 1502.

Cells·2026
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.8K

Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes.

Moshe Naoz1, Nir S Gov1

  • 1Department of Chemical and Biological Physics, Weizmann Institute of Science, P.O.B. 26, Rehovot 76100, Israel.

Cells
|March 27, 2020
PubMed
Summary
This summary is machine-generated.

Cellular membrane proteins that curve and recruit actin polymerization create distinct dynamics on the basal versus dorsal cell sides. Substrate confinement transforms dorsal protrusions into wave-like structures on the basal side.

Keywords:
actin wavescurved proteinsdynamic instabilitypodosomes

More Related Videos

Reconstitution of Actin-Based Motility with Commercially Available Proteins
08:40

Reconstitution of Actin-Based Motility with Commercially Available Proteins

Published on: October 28, 2022

2.2K
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
09:09

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

2.8K

Related Experiment Videos

Last Updated: Dec 25, 2025

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.8K
Reconstitution of Actin-Based Motility with Commercially Available Proteins
08:40

Reconstitution of Actin-Based Motility with Commercially Available Proteins

Published on: October 28, 2022

2.2K
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
09:09

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

2.8K

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Cells adhered to solid substrates display unique actin dynamics on their basal membranes compared to dorsal membranes.
  • Curved membrane proteins and protein complexes recruit actin polymerization when the cell membrane is confined by a substrate.

Purpose of the Study:

  • To investigate the dynamics of curved membrane proteins that recruit actin polymerization under substrate confinement.
  • To understand how membrane curvature and actin forces contribute to distinct cellular behaviors on basal and dorsal cell sides.

Main Methods:

  • Utilized biophysical models to explore protein-membrane interactions.
  • Simulated actin polymerization dynamics influenced by membrane curvature and substrate confinement.
  • Analyzed the formation and propagation of membrane structures.

Main Results:

  • Curved proteins can induce membrane protrusions on the dorsal side.
  • On the basal side, substrate confinement limits protrusion extension, leading to wave-like structures.
  • Adhesion molecules stabilize localized protrusions, resembling podosomes.

Conclusions:

  • The coupling of membrane curvature and actin forces explains the differential actin-membrane dynamics observed between the basal and dorsal sides of adhered cells.
  • Substrate-induced constraints play a critical role in shaping cellular protrusions and their dynamics.