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Reversed-phase thin-layer chromatography used to study the simultaneous effect of pH and ion strength on the
T Cserháti1, M Szögyi, L Lelkes
1Plant Protection Institute, Hungarian Academy of Sciences, Budapest.
Journal of Biochemical and Biophysical Methods
|August 1, 1988
Abstract:
The effect of ion strength and pH value of the eluent on the determination of the lipophilicity of chlorhexidine was studied by reversed-phase thin-layer chromatography. The method has been improved by using various buffers: aqueous solutions of formic, acetic and propionic acids and their sodium salts in different ratios and in various concentrations. Stepwise regression analysis separated the effect of pH value, ion strength and acid type on the lipophilicity of chlorhexidine and proved that the ion strength exerted a higher impact than the pH value did. The effect of alkyl chain length of the acids was of secondary importance.