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LncRNA-SNHG16 Silencing Inhibits Prostate Carcinoma Cell Growth, Downregulate GLUT1 Expression and Reduce Glucose

Mingfeng Shao1, Ziqiang Yu1, Jianan Zou1

  • 1Department of Urology, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei City, Anhui Province 230031, People's Republic of China.

Cancer Management and Research
|March 27, 2020
PubMed
Abstract

Insights

Long non-coding RNA SNHG16 is overexpressed in prostate carcinoma, correlating with glucose transporter 1 (GLUT-1) and inhibiting cancer cell proliferation. SNHG16 may be a therapeutic target for prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Long non-coding RNA SNHG16 (lncRNA-SNHG16) is implicated as an oncogene in various cancers.
  • Its specific role in prostate carcinoma pathogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of lncRNA-SNHG16 in prostate carcinoma.
  • To determine the relationship between lncRNA-SNHG16, glucose transporter 1 (GLUT-1), and prostate cancer cell proliferation.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) was used to analyze lncRNA-SNHG16 and GLUT-1 expression in prostate carcinoma and normal tissues.
  • In vitro transfections and cell proliferation assays were conducted to assess gene interactions and cellular behavior.
  • RNA interference (siRNA) was employed to silence lncRNA-SNHG16 expression.

Main Results:

  • lncRNA-SNHG16 overexpression significantly differentiated prostate carcinoma from normal tissues.
  • Positive correlation observed between lncRNA-SNHG16 and GLUT-1 mRNA levels in prostate carcinoma.
  • Silencing lncRNA-SNHG16 reduced GLUT-1 expression, glucose uptake, and inhibited prostate cancer cell proliferation without affecting normal prostate cells.

Conclusions:

  • lncRNA-SNHG16 plays a crucial role in promoting prostate carcinoma progression.
  • lncRNA-SNHG16 emerges as a potential therapeutic target for prostate cancer treatment.
Keywords:
SNHG16glucose transporter 1prostate carcinoma

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