Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+-activated K+ currents in Necturus choroid plexus.

D D Loo1, P D Brown, E M Wright

  • 1Department of Physiology, UCLA School of Medicine 90024-1751.

The Journal of Membrane Biology
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Searching for Chameleon Dark Energy with Mechanical Systems.

Physical review letters·2022
Same author

Universal long-wavelength nonlinear optical response of noble gases.

Optics express·2019
Same author

Superradiant Amplification of Acoustic Beams via Medium Rotation.

Physical review letters·2019
Same author

Memory effects in the long-wave infrared avalanche ionization of gases: a review of recent progress.

Reports on progress in physics. Physical Society (Great Britain)·2019
Same author

Rotation-dependent nonlinear absorption of orbital angular momentum beams in ruby.

Optics letters·2018
Same author

Self-Channeling of High-Power Long-Wave Infrared Pulses in Atomic Gases.

Physical review letters·2017
Same journal

Structural Basis of SERCA Inhibition by Derivatives of di-tert-butylhydroquinone Revealed by X-ray Crystallography.

The Journal of membrane biology·2026
Same journal

Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation.

The Journal of membrane biology·2026
Same journal

Polyproline Modulates Membrane Translocation of Arginine-Rich Cell-Penetrating Peptides: Insights from Molecular Dynamics Simulations.

The Journal of membrane biology·2026
Same journal

Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses.

The Journal of membrane biology·2026
Same journal

Protonation of Key Acidic Residues Reveals Binding Features of PCABs to Gastric H, K-ATPase.

The Journal of membrane biology·2026
Same journal

Electrostatic Interaction as a Key Modulator of Na<sup>+</sup>,K<sup>+</sup>-ATPase Function.

The Journal of membrane biology·2026
See all related articles

This study identifies calcium-activated potassium channels in Necturus choroid plexus, crucial for maintaining intracellular potassium during cerebrospinal fluid secretion.

Area of Science:

  • Physiology
  • Cell Biology
  • Neuroscience

Background:

  • The Necturus choroid plexus epithelium plays a vital role in cerebrospinal fluid (CSF) secretion.
  • Understanding ion channel activity is essential for elucidating the mechanisms of CSF production.

Purpose of the Study:

  • To investigate the properties of voltage-dependent outward currents in Necturus choroid plexus epithelial cells.
  • To identify the specific ion channels responsible for these currents and their role in cellular function.

Main Methods:

  • Tight-seal whole-cell recording technique was employed to measure cell potential and resistance.
  • Voltage-clamp protocols were used to activate and characterize outward currents.
  • Ion substitution experiments and varying calcium concentrations were performed to determine channel selectivity and activation kinetics.

Related Experiment Videos

Main Results:

  • A cell potential of -59 ± 2 mV and whole-cell resistance of 56 ± 6 MΩ were recorded.
  • Depolarizing potentials activated delayed outward currents, suggesting Ca2+-activated K+ channels.
  • These currents exhibited high K+ selectivity, were modulated by intracellular Ca2+ and membrane potential, and contributed significantly to cell conductance.

Conclusions:

  • The delayed outward currents are identified as K+ currents flowing through Ca2+-activated K+ channels.
  • These channels play a significant role in regulating intracellular K+ concentrations, particularly during stimulated CSF secretion.
  • The findings provide insights into the electrophysiological mechanisms underlying choroid plexus function.