Related Experiment Video
Updated: Dec 25, 2025
09:50
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
Published on: October 27, 2019
7.4K
A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy
D E Skilling1, J E Barlough1, E S Berry1
1Calicivirus Research Laboratory, College of Veterinary Medicine, Oregon State University, 97331-4802 Corvallis, Oregon USA.
Abstract:
A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus types differing in size/shape and buoyant density.
