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Study of measles virus recombinant proteins and their immunobiological properties
M V Kulak1, N A Netesova1, P A Belavin1
1Vector State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk oblast, Russia.
Abstract:
Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. BALB/c mice were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune sera against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be capable of neutralizing the virus in titer 1 : 13.5 and 1 : 22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1 : 25.7.
Insights
Researchers developed recombinant measles virus proteins (nucleocapsid and hemagglutinin) and tested their immunobiological properties. These proteins generated antibodies capable of neutralizing the measles virus, showing potential for diagnostic and therapeutic applications.
Area of Science:
- Virology
- Immunology
- Biotechnology
Background:
- Measles virus (genotype A, strain NovO/96) poses a significant public health challenge.
- Understanding the immunogenic properties of measles virus proteins is crucial for vaccine development and diagnostics.
- Recombinant protein technology offers a platform for producing specific viral antigens for immunological studies.
Purpose of the Study:
- To produce and characterize recombinant nucleocapsid (rN) and hemagglutinin (rH/Nh) proteins of the measles virus strain NovO/96.
- To evaluate the immunobiological properties of these recombinant proteins.
- To assess the neutralizing capacity of antibodies generated against these recombinant proteins.
Main Methods:
- Production of recombinant measles virus nucleocapsid (rN) and hemagglutinin (rH/Nh) proteins.
- Immunization of BALB/c mice with recombinant proteins and native antigen.
- Analysis of hyperimmune sera using enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization (PRN) assays.
Main Results:
- Hyperimmune sera against both recombinant proteins (rN, rH/Nh) and native antigen demonstrated high activity in ELISA.
- Antibodies against rN and rH/Nh proteins exhibited measles virus neutralizing activity with titers of 1:13.5 and 1:22.9, respectively.
- Antibodies generated against the native antigen showed a neutralization titer of 1:25.7.
Conclusions:
- Recombinant measles virus proteins rN and rH/Nh are immunogenic and elicit a neutralizing antibody response.
- The study validates the potential of using these recombinant proteins for developing diagnostic tools or subunit vaccines against measles.
- The findings contribute to the understanding of measles virus immunogenicity and provide a basis for further research in measles control.
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