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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
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Pixel-level aflatoxin detecting in maize based on feature selection and hyperspectral imaging.

Jiyue Gao1, Jiangong Ni1, Dawei Wang1

  • 1School of Science and Information, Qingdao Agricultural University, Qingdao, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|March 29, 2020
PubMed
Summary

Hyperspectral imaging can detect aflatoxin in maize, a potent liver carcinogen. Feature selection methods like Relieff combined with Random Forest achieved 99.38% accuracy, while using all bands reached 100% accuracy.

Keywords:
AflatoxinClassificationCornFeature extractHyperspectral

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Area of Science:

  • Agricultural Science
  • Food Safety
  • Spectroscopy

Background:

  • Aflatoxins are highly toxic fungal metabolites commonly found in maize, posing a significant risk of liver cancer.
  • Accurate detection of aflatoxin contamination is crucial for food safety and public health.

Purpose of the Study:

  • To develop and evaluate hyperspectral imaging models for pixel-level aflatoxin classification in maize.
  • To compare the effectiveness of different feature extraction and selection methods for improving classification accuracy.

Main Methods:

  • Utilized hyperspectral data with 600 bands per pixel, labeled as 'clean' or 'contaminated'.
  • Applied three feature extraction/selection approaches: 4 specific bands, Principal Component Analysis (PCA), and algorithms (Fscnca, Fscmrmr, Relieff, Fishier) for top 10 band selection.
  • Classified pixels using Random Forest (RF) and K-nearest neighbor (KNN) algorithms with selected/extracted features.

Main Results:

  • The Relieff feature selection method achieved the highest accuracy (99.38% with RF, 98.77% with KNN).
  • PCA feature extraction with RF yielded 93.83% accuracy.
  • Using all 600 bands without feature extraction resulted in 100% accuracy, though potentially computationally intensive.
  • Bands selected from prior studies achieved 89.51% accuracy.

Conclusions:

  • Feature extraction and selection methods significantly impact aflatoxin classification accuracy using hyperspectral data.
  • The Relieff algorithm combined with RF offers a highly accurate and efficient approach for aflatoxin detection.
  • Full-band hyperspectral data provides the highest accuracy for aflatoxin classification when computational time is not a constraint.