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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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The STEME system: a novel tool for directed evolution in vivo.

Feng Yue Hu1, Ke Jian Wang1

  • 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.

Yi Chuan = Hereditas
|March 29, 2020
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Summary

Scientists engineered novel saturated targeted endogenous mutagenesis editors (STEMEs) for in vivo directed evolution in plants. This breakthrough enables rapid development of crop traits, like herbicide resistance in rice.

Keywords:
STEMEdirected evolutiondouble base editinggermplasm innovation

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Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genetics

Background:

  • Directed evolution is crucial for protein engineering and developing crop traits.
  • Existing methods have limitations in inducing diverse mutations for trait selection.

Purpose of the Study:

  • To engineer novel saturated targeted endogenous mutagenesis editors (STEMEs) for in vivo directed evolution in plants.
  • To demonstrate the application of STEMEs in creating herbicide-resistant rice.

Main Methods:

  • Integrated two single-base editing techniques to create STEMEs.
  • Induced C:G>T:A and A:T>G:C double-base editing in plants.
  • Targeted the OsACC carboxyltransferase domain coding sequence for artificial evolution.

Main Results:

  • Successfully achieved in vivo directed evolution and function selection in plants.
  • Engineered herbicide-resistant rice through targeted gene editing.
  • Demonstrated efficient induction of double-base editing (C:G>T:A and A:T>G:C).

Conclusions:

  • The STEME system offers a powerful new tool for in vivo gene directed evolution in plants.
  • This technology has significant potential for agronomic trait screening and functional gene research.
  • STEMEs can accelerate innovation in crop germplasm resources.