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Characterization of the Enterobacter Phage vB_EclM_CIP9

Klara Wang1,2, Marielou G Tamayo1, Tiffany V Penner2

  • 1Cytophage Technologies, Inc., Winnipeg, Manitoba, Canada.

Microbiology Resource Announcements
|March 29, 2020
PubMed

Insights

A new bacteriophage, vB_EclM_CIP9, has been identified that targets multidrug-resistant Enterobacter cloacae. This discovery offers a potential new strategy for combating hospital-acquired infections caused by this opportunistic pathogen.

Area of Science:

  • Microbiology
  • Virology
  • Infectious Diseases

Background:

  • Enterobacter cloacae is an opportunistic pathogen responsible for hospital-acquired infections, particularly in immunocompromised individuals.
  • The rise of multidrug-resistant (MDR) bacterial strains poses a significant threat to public health, complicating treatment options.

Purpose of the Study:

  • To describe a novel bacteriophage, vB_EclM_CIP9, with the capacity to infect and potentially control MDR Enterobacter cloacae.
  • To characterize the genomic and morphological features of the newly discovered phage.

Main Methods:

  • Isolation and characterization of bacteriophage vB_EclM_CIP9 from environmental samples.
  • Genomic sequencing and analysis of the bacteriophage.
  • Identification of predicted open reading frames within the phage genome.

Main Results:

  • Discovery of vB_EclM_CIP9, a novel myovirus bacteriophage.
  • vB_EclM_CIP9 infects a multidrug-resistant isolate of Enterobacter cloacae.
  • The phage possesses a large genome of 174,924 base pairs with 296 predicted open reading frames.

Conclusions:

  • Bacteriophage vB_EclM_CIP9 represents a promising candidate for phage therapy against MDR Enterobacter cloacae infections.
  • Further research into the lytic activity and host range of vB_EclM_CIP9 is warranted.

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