Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.1K
Redox Titration: Overview01:21

Redox Titration: Overview

4.7K
Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
4.7K
Redox Titration: Other Oxidizing and Reducing Agents01:26

Redox Titration: Other Oxidizing and Reducing Agents

1.3K
Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
1.3K
Redox Reactions01:24

Redox Reactions

58.0K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
58.0K
Redox Titration: Iodimetry and Iodometry01:23

Redox Titration: Iodimetry and Iodometry

5.3K
Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
5.3K
Hydroboration-Oxidation of Alkenes03:08

Hydroboration-Oxidation of Alkenes

10.8K
In addition to the oxymercuration–demercuration method, which converts the alkenes to alcohols with Markovnikov orientation, a complementary hydroboration-oxidation method yields the anti-Markovnikov product. The hydroboration reaction, discovered in 1959 by H.C. Brown, involves the addition of a B–H bond of borane to an alkene giving an organoborane intermediate. The oxidation of this intermediate with basic hydrogen peroxide forms an alcohol.
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution of allostery without shape shifting: Internal dynamics drives functional diversification of a transcriptional repressor superfamily.

bioRxiv : the preprint server for biology·2026
Same author

Mitochondria-targeted metformin analogs activate the ER stress-unfolded protein response pathway to drive apoptosis in pancreatic cancer.

Cell death & disease·2026
Same author

Monoclonal antibodies against nitrated nerve growth factor reveal an oxidation-dependent pathogenic hallmark in ALS.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multifaceted roles for persulfide species in redox chemical biology.

Nature chemical biology·2026
Same author

Microbial Metabolism of Levodopa as an Adjunct Therapeutic Target in Parkinson's Disease.

Antioxidants (Basel, Switzerland)·2026
Same author

Toward Specific Detection of Peroxynitrite─Intramolecular Cyclization of Boronobenzylated Pyridinium Probe in Chemical and Cellular Systems.

Chemical research in toxicology·2026

Related Experiment Video

Updated: Dec 25, 2025

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Published on: September 17, 2021

4.6K

Tracking isotopically labeled oxidants using boronate-based redox probes.

Natalia Rios1,2, Rafael Radi1,2, Balaraman Kalyanaraman3

  • 1Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11800 Montevideo, Uruguay.

The Journal of Biological Chemistry
|March 29, 2020
PubMed
Summary

Boronate probes can track reactive oxygen and nitrogen species by incorporating oxygen atoms from oxidants like hydrogen peroxide. This allows for precise identification of these harmful molecules in biological systems.

Keywords:
hydrogen peroxidehypochlorous acidisotope tracingisotopic tracermass spectrometry (MS)oxygen radicalsoxygen-18peroxymonocarbonateperoxynitritereaction mechanismreactive nitrogen species (RNS)reactive oxygen species (ROS)redox probessuperoxide ionsuperoxide radical anion

More Related Videos

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

2.1K
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.4K

Related Experiment Videos

Last Updated: Dec 25, 2025

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Published on: September 17, 2021

4.6K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

2.1K
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.4K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Probes

Background:

  • Reactive oxygen and nitrogen species (RONS) are implicated in numerous diseases.
  • Detecting short-lived RONS requires molecular probes forming stable products.
  • Current probe mechanisms are often poorly understood, limiting applications.

Purpose of the Study:

  • To investigate the oxygen atom source in phenylboronate oxidation products.
  • To determine if boronates can trace specific oxidants using isotopic labeling.
  • To understand the mechanism of boronate probe conversion to oxidant-specific products.

Main Methods:

  • Oxygen-18 labeling mass spectrometry (MS) study.
  • Phenylboronate probe targeted to mitochondria.
  • Oxidation by hydrogen peroxide, peroxymonocarbonate, hypochlorite, and peroxynitrite.

Main Results:

  • Boronate oxidation incorporated oxygen atoms directly from the tested oxidants.
  • This confirms boronates can serve as probes for isotopically labeled oxidants.
  • Specific product detection can identify biological oxidants.

Conclusions:

  • Boronates can be utilized to track specific oxidants in biological systems.
  • This provides a method for precise identification of RONS.
  • Understanding probe mechanisms enhances their application in disease research.